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| Francisco,Flávio de O.; Brito,Rute M.; Arias,Maria C.. |
| In the present study we compare genetic characteristics (allele diversity and observed heterozygosity) of microsatellite loci, from three stingless bee species (Plebeia remota Holmberg, Partamona mulata Moure In Camargo and Partamona helleri Friese), amplified by using heterospecific primers originally designed for Melipona bicolor Lepeletier and Scaptotrigona postica Latreille. We analyzed 360 individuals of P. remota from 72 nests, 58 individuals of P. mulata from 58 nests, and 47 individuals of P. helleri from 47 nests. The three species studied showed low level of polymorphism for the loci amplified with primers derived from M. bicolor. However, for the loci amplified with primers derived from S. postica, only P. remota presented low level of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Plebeia remota; Partamona mulata; Partamona helleri; Heterozygosity; Polymorphism. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2006000500011 |
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| Moretto,Geraldo; Arias,Maria C.. |
| The endemic range of the stingless bee Melipona quadrifasciata Lepeletier extends from the southern most state in Brazil, Rio Grande do Sul, up to the state of Paraíba, where it originally inhabited the Atlantic Rain Forest. Two subspecies have been morphologically recognized based on the tergal band pattern (yellow stripes across the abdomen), M. q. quadrifasciata and M. q. anthidioides. The objective of the present study was to characterize restriction site variation in the mitochondrial DNA of each subspecies. Four colonies of each subspecies were sampled. One individual per colony was used for DNA extraction and further analysis through amplification of nine fragments of the mitochondrial genome and digestion with 13 restriction enzymes (PCR+RFLP).... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Restriction enzyme; Polymorphism; PCR+RLFP; MtDNA. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000300004 |
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